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BIOCOUNT® 7000
World's first Reader for 8-well strips
The Biocount® 7000 is a special system for fast routine applications and environments and it can analyze 8-well strips in about 15-20 seconds.
Same illumination advantages as the Bioreader®:
- Very good differentiation between 'real' ELISpots and non-specific objects.
- Best separation of ELISpot clusters.
- Supresses reflections on the well border.
Operation via touchscreen.
Loading the strip and/or well descriptions (like patient IDs) usually by use of barcodes or via a LIS/LIMS import file.
Data export via PDF, MS Excel or CSV. Image export via PDF or MS Excel.
Quick access to the daily journal. Print or PDF.
Further reports with possibility to filtert he results by date, well description and measure operator.
Very small foot print, only 24 x 20 x 30 cm (width x depth x height).
EC declaration of conformity available.
Protected design: EU 015002986-0001 and USA: 29/861,617.
Dimension
Elispot - Enzymatic single color
Blue, red, green or silver substrat Elispot.
Detection of antibodies. Enzyme-conjugated and precipitating substrate.
"The enzyme-linked immune absorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell."
[https://en.wikipedia.org/wiki/ELISpot]
Why Elispot?
The cytokine Elispot assay is designed to enumerate cytokine secreting cells in single cell suspensions of lymphoid tissue, CNS tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMC).
The assay has the advantage of detecting only activated/memory T cells and the cytokine release can be detected at the single cell level, allowing direct determination of T cell frequencies.
The ELISPOT assay is an effective tool to enumerate antigen-specific T cells in the circulation of immunized humans and animals at much lower frequencies than possible with other currently available methods.
The ELISPOT assay has proven to be a sensitive and unique system to follow disease progression in human individuals or animals. Several studies have indicated that alterations in the frequency of cytokine pc in different compartments of the body adequately reflect changes in immune function.
The ELISPOT assay may be used to determine effects of drugs, chemicals or other compounds on cytokine secretion in vitro, thereby providing data on their putative modulatory effects on immune function in vivo
The ELISPOT assay is currently being used increasingly for the quantitative assessment of peptide reactive T lymphocytes from PBMC in infectious disease (3, 9) or in the course of vaccination trials aimed at the induction of tumor-specific T cells in patients with cancer.
Elispot may be used for Diagnosis of genetic defects,Allergic diseases,Autoimmune diseases,Transplantation,Cancer research,Acute inflammation,Acute infectious diseases and septic shock.
